Which of the following is not a product of recombinant DNA technology?

The correct answer is Dolly.

Concept:

• Recombinant DNA technology is the process of combining DNA molecules from two different organisms and inserting them into a host organism to create new genetic combinations.
• Process of Recombinant DNA technology
• Recombinant DNA technology involves the following steps:
  • Isolation of DNA.
  • Fragmentation of DNA by restriction endonucleases.
  • isolation of a desired DNA fragment.
  • Amplification of the gene of interest.
  • ligation of the DNA fragment into a vector.
  • Insertion of recombinant DNA into the host.
  • Culturing the host cells on a suitable medium at a large scale.
  • Extraction of the desired gene product.
  • Downstream processing of the products as finished product, ready for marketing.

Explanation:

• Recombinant DNA technology is widely used in agriculture to create genetically modified organisms such as Flavr Savr tomatoes, and Bt-cotton to protect the plant from ball worms, among other things.
• Cloning is the production of an exact copy of a cell, another living part, or an entire organism.
• Cloning of an animal was successfully performed for the first time by Ian Wilmut and his colleagues at the Roslin Institute in Edinburgh, Scotland. 
• They successfully cloned a sheep named Dolly.
• Dolly, the first cloned mammal, was born on July 5, 1996.

Thus, Dolly is not a product of recombinant DNA technology.

Additional InformationGlo-fish:

• Glo-fish are genetically modified fish created with Recombinant DNA technology.

Bt-cotton:

• Some strains of Bacillus thuringiensis produce proteins that kill some insects like lepidopterans (tobacco budworm, armyworm), coleopterans (beetles) and dipterans (flies, mosquitoes).
• Bacillus thuringiensis forms protein crystals which contain toxic insecticidal protein.

Flavr savr:

• Flavr Savr, a genetically modified tomato, was the first commercially grown genetically engineered food to receive a human consumption licence.